Journal: International Journal of Molecular Sciences

Article Title: Rheumatoid Arthritis-Associated MicroRNA-155 Targets SOCS1 and Upregulates TNF-α and IL-1β in PBMCs

doi: 10.3390/ijms141223910

Figure Lengend Snippet: Demographic, clinical, and laboratory data of patients with rheumatoid arthritis, osteoarthritis, and controls.

Article Snippet: The levels of TNF-α and IL-1β in plasma and PBMCs supernatant were measured by an enzyme immunoassay using the human TNF-α and IL-1β ELISA Kit (Sino Biological Inc., Beijing, China).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Rheumatoid Arthritis-Associated MicroRNA-155 Targets SOCS1 and Upregulates TNF-α and IL-1β in PBMCs

doi: 10.3390/ijms141223910

Figure Lengend Snippet: miR-155 upregulation correlates with increased production of TNF-α and IL-1β in Rheumatoid arthritis (RA) patients. ( A ) and ( B ): Increased expression of TNF-α and IL-1β in RA plasma. Results are expressed as pg/mL by an ELISA test; ( C ): MiR-155 is upregulated in PBMCs of active RA patients. Results in patients with RA ( n = 45) and OA ( n = 32) are shown as fold increase relative to the control group ( n = 25); ( D ) and ( E ): Correlations between Relative miR-155 expression with the TNF-α or IL-1β expression. RA, rheumatoid arthritis; OA, osteoarthritis. Statistical significance was considered with a p value < 0.05.

Article Snippet: The levels of TNF-α and IL-1β in plasma and PBMCs supernatant were measured by an enzyme immunoassay using the human TNF-α and IL-1β ELISA Kit (Sino Biological Inc., Beijing, China).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: International Journal of Molecular Sciences

Article Title: Rheumatoid Arthritis-Associated MicroRNA-155 Targets SOCS1 and Upregulates TNF-α and IL-1β in PBMCs

doi: 10.3390/ijms141223910

Figure Lengend Snippet: Correlations between miR-155 expression and the various clinical and laboratory data of patients with rheumatoid arthritis ( n = 45).

Article Snippet: The levels of TNF-α and IL-1β in plasma and PBMCs supernatant were measured by an enzyme immunoassay using the human TNF-α and IL-1β ELISA Kit (Sino Biological Inc., Beijing, China).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Rheumatoid Arthritis-Associated MicroRNA-155 Targets SOCS1 and Upregulates TNF-α and IL-1β in PBMCs

doi: 10.3390/ijms141223910

Figure Lengend Snippet: miR-155 promotes production of TNF-α and IL-1β in human PBMCs. ( A ) Fold changes of miR-155 expression were determined by qRT-PCR after the transfection of miR-control, or miR-155 mimics at 25 or 50 nM for 24 h; ( B ) and ( C ) Expression of TNF-α and IL-1β in supernatant of PBMCs 24 h post LPS treatment, or 24 h post transfection with 25 nM miR-155 mimics, 50 nM miR-155 mimics or 50 nM miRNA control. Results were expressed as pg/mL by an ELISA test; and ( D ) mRNA expression of TNF-α (group 1) and IL-1β (group 2) in PBMCs 24 h after LPS treatment or 24 h post transfection with 25 nM miR-155 mimics, 50 nM miR-155 mimics or 50 nM miRNA control. All results are the average of three independent experiments. Statistical significance is shown.

Article Snippet: The levels of TNF-α and IL-1β in plasma and PBMCs supernatant were measured by an enzyme immunoassay using the human TNF-α and IL-1β ELISA Kit (Sino Biological Inc., Beijing, China).

Techniques: Expressing, Quantitative RT-PCR, Transfection, Enzyme-linked Immunosorbent Assay

Journal: International Journal of Molecular Sciences

Article Title: Rheumatoid Arthritis-Associated MicroRNA-155 Targets SOCS1 and Upregulates TNF-α and IL-1β in PBMCs

doi: 10.3390/ijms141223910

Figure Lengend Snippet: miR-155 inhibitor reduces the production of proinflammatory cytokines induced by LPS. ( A ) Fold changes in miR-155 expression were determined by RT-qPCR 24 h post transfection with 50 nM miRNA inhibitor control, 25 or 50 nM miR-155 inhibitor; ( B ) and ( C ) Expression of TNF-α and IL-1β in supernatant of PBMCs 24 h after LPS treatment, or (and) 24 h post transfection with 5 nM miR-155 inhibitor or 10 nM miR-155 inhibitor. Results were expressed as pg/mL by an ELISA test; and ( D ) mRNA expression of TNF-α (group 1) and IL-1β (group 2) in PBMCs 24 h after LPS treatment, or (and) 24 h post transfection with 10 nM miRNA inhibitor control, 5 nM miR-155 inhibitor or 10 nM miRNA inhibitor. All results are the average of three independent experiments. Statistical significance is shown.

Article Snippet: The levels of TNF-α and IL-1β in plasma and PBMCs supernatant were measured by an enzyme immunoassay using the human TNF-α and IL-1β ELISA Kit (Sino Biological Inc., Beijing, China).

Techniques: Expressing, Quantitative RT-PCR, Transfection, Enzyme-linked Immunosorbent Assay